P8825

Immunofluorescence
Human Tonsil stained with Anti-CD3, T Cell (Cat. No. C7930) at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen (Cat. No. P8825) at a 1:500 dilution (green). Counterstained with DAPI.
From Pamela Wirth, Plastic Surgery Department, Vanderbilt University, Nashville, TN.

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P8825

Sigma

 

Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse

clone PC 10, ascites fluid

Synonym:Anti-PCNA, Monoclonal Anti-Proliferating Cell Nuclear Antigen

Description

ImmunogenPCNA-Protein A fusion protein
SpecificityRecognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues.

Properties

antibody formascites fluid
clonePC 10 (monoclonal)
contains15 mM sodium azide
species reactivityhuman, insect, monkey, yeast
application(s)flow cytometry: suitable
 immunoblotting: suitable
 immunocytochemistry: suitable
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections
 immunohistochemistry (frozen sections): suitable
 immunoprecipitation: suitable
 indirect ELISA: suitable
 microarray: suitable
isotypeIgG2a
Gene Informationhuman ... PCNA(5111)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

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References

referenceJiang, Z., Activation of retinoblastoma protein in mammary gland leads to ductal growth suppression, precocious differentiation, and adenocarcinoma J. Cell Biol. 156, 185-198, (2002) Abstract
 Beuvink, I., et al., Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands J. Cell Biol. 161, 583-592, (2003) Abstract
 Anderson, L.A., and Perkins, N.D., Regulation of RelA (p65) Function by the Large Subunit of Replication Factor C Mol. Cell. Biol. 23, 721-732, (2003) Abstract
 Waseem, N.H., and Lane, D.P., Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J. Cell Sci. 96, 121-129, (1990)
 Hall, P.A., et al., Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J. Pathol. 162, 285-294, (1990)
 Harrison, R.F., et al., Immunohistochemical evidence for the expression of proliferating cell nuclear antigen (PCNA) by non-proliferating hepatocytes adjacent to metastatic tumours and in inflammatory conditions. J. Pathol. 171, 115-122, (1993)
 Start, R.D., et al., Delay in fixation does not affect the immunoreactivity of proliferating cell nuclear antigen (PCNA). J. Pathol. 168, 197-199, (1992)
 Cattoretti, G., et al., Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171, 83-98, (1993)
 Wolf, H.K., and Dittrich, K.L., Detection of proliferating cell nuclear antigen in diagnostic histopathology. J. Histochem. Cytochem. 40, 1269-1273, (1992)
 Yu, C.C.-W, et al., The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem. J. 24, 121-131, (1992)
 Jones, H.B., et al., Phenobarbital-induced hepatocellular proliferation: anti-bromodeoxyuridine and anti-proliferating cell nuclear antigen immunocytochemistry. J. Histochem. Cytochem. 41, 21-27, (1993)